Cytotoxicity assays play a crucial role in drug discovery, toxicology studies, and biomedical research. These in vitro assays provide valuable insights into the potential toxic effects of substances on living cells. By measuring the viability and metabolic activity of cells, researchers can evaluate the safety profile of various compounds and develop a better understanding of their mechanism of action. This article aims to explore the fundamentals of cytotoxicity assays, highlighting their importance and key methodologies while emphasizing the in vitro nature of these tests. Importance of Cytotoxicity Assays Cytotoxicity assays are essential for assessing the potential harmful effects of chemicals, pharmaceuticals, and other substances on human health. By utilizing in vitro assays, researchers can evaluate the cytotoxic effects of compounds before proceeding to in vivo studies, thereby minimizing risks associated with animal testing and facilitating the identification of potential ther...
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